Ly6a Rabbit Polyclonal Antibody (FITC)

SKU: orb9766

Description

Ly6a Rabbit Polyclonal Antibody (FITC) is an FITC conjugated antibody targeting Ly6a. This antibody is suitable for FC, IF. It exhibits reactivity with Mouse, Rat samples.

Research Area

Developmental Biology, Hematopoietic Stem Cell Markers, Hematopoietic System, Molecular Biology, Stem Cell Research, Surface Molecules

Images & Validation

• Tested Applications: FC, IF
• Dilution Range: Flow-Cyt=2μg /Test, IF=1:100-500
• Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse Sca1/Ly6A/E (15-110/134aa)
• Target: Ly6a
• Purification: Affinity purified by Protein A
• Conjugation: FITC

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Stem cell antigen 1; Sca1/Ly6A/E; SCA-1; T-cell-activating protein; TAP; Ly6al; Sca1; LY6A_MOUSE.

Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-Sca1/FITC Conjugated antibody (orb9766), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Mouse thymus. Primary Antibody (green line): Rabbit Anti-Sca1 antibody (orb9766), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Rat spleen. Primary Antibody (green line): Rabbit Anti-Sca1 antibody (orb9766), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

Ly6a

Cheng, Tingting et al. Lung-resident mesenchymal stem cells regulated the inflammatory responses in innate and adaptive immune cells through HVEM-BTLA pathway during ARDS Exp Cell Res, 395, 112155 (2020)

PubMed: 32598875