LXR alpha + LXR beta Rabbit Polyclonal Antibody

SKU: orb185093

Description

LXR alpha + LXR beta Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cell Biology, Cholesterol Metabolism, Lipid Metabolism, Lipids and Lipoproteins Metabolism, Metabolism, Signal Transduction

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=2ug/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Rat, Xenopus

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LXR alpha (51-150/447aa)
• Target: NR1H3
• Molecular Weight: 51 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Liver X receptor alpha; Liver X receptor alpha; Liver X receptor beta; LXR a; LXR; LXR b; LXRa; LXRalpha; LXRb; LXRbeta; NER; NERI; NR1H2; NR1H3; Nuclear orphan receptor LXR alpha; Nuclear orphan receptor LXR beta; Nuclear receptor NER; Nuclear receptor subfamily 1 group H member 2; Nuclear receptor subfamily 1 group H member 3; Oxysterols receptor LXR alpha; Oxysterols receptor LXR beta; RIP15; RLD1; Ubiquitously expressed nuclear receptor; UNR.

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-LXR alpha + LXR beta antibody (orb185093), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-LXR alpha + LXR beta (orb185093) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.

Quick Database Links

Gene Symbol

NR1H3

UniProt

Q13133