LULL1/TOR1AIP2 Rabbit Polyclonal Antibody

• Catalog Number: orb184866
• Category: Antibodies
• Description: LULL1/TOR1AIP2 Rabbit Polyclonal Antibody
• Target: TOR1AIP2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human LULL1 (221-320/470aa)
• UniProt ID: Q8NFQ8
• MW: 51 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TOR1AIP2; IFRG15; Interferon responsive gene 15; L
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (LULL1) Polyclonal Antibody, Unconjugated (orb184866) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (LULL1) Polyclonal Antibody, Unconjugated (orb184866) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: K562 (Human) Cell Lysate at 30 ug, Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 3: Hela (Human) Cell Lysate at 30 ug, Lane 4: HUVEC (Human) Cell Lysate at 30 ug, Lane 5: U2os (Human) Cell Lysate at 30 ug, Lane 6: HepG2 (Human) Cell Lysate at 30 ug, Lane 7: Liver (Mouse) Lysate at 40 ug, Lane 8: Liver (Rat) Lysate at 40 ug, Lane 9: Kidney (Mouse) Lysate at 40 ug, Lane 10: Cerebrum (Mouse) Lysate at 40 ug, Lane 11: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-LULL1/TOR1AIP2 (orb184866) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51kD.

Sample: Placenta (Mouse) Lysate at 40 ug, Large intestine (Mouse) Lysate at 40 ug, Primary: Anti-LULL1/TOR1AIP2 (orb184866) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51kD, Observed band size: 56kD.

Sample: Small intestine (Mouse) Lysate at 40 ug, Primary: Anti-LULL1/TOR1AIP2 (orb184866) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51kD, Observed band size: 56kD.