LTA Antibody

• Catalog Number: orb1671867
• Category: Antibodies
• Description: LTA Antibody
• Clonality: Recombinant
• Clone Number: 3F12.2D3
• Tested applications: ELISA, FC, NeA
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTα expressed in E. coli - the hybridoma clone was selected for its ability to bind LTα3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: LTA
• UniProt ID: P01374
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: LTα3 chimeric 3F12,LT,, LTα, Tumor necrosis factor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-Lymphotoxin alpha antibody 3F12.2D3. Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35 ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the r-chimeric version of 3F12.2D3 (orb1671867) at 2 ug/ml before detection using an anti-rondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3kDa- tho ugh the form shown here is likely glycosylated. orb1671867 successfully detected Lymphotoxin alpha in LPS-treated human PBM samples.

Flow-cytometry using the anti-Lymphotoxin alpha antibody 3F12.2D3. U937 cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with unimmunized r antibody (MOPC-21; isotype control - black line) or the r-chimeric version of 3F12.2D3 (orb1671867 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.