LSM4 Antibody

• Catalog Number: orb1257266
• Category: Antibodies
• Description: LSM4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-139 of human LSM4 (NP_036453.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 15kDa
• Target: LSM4
• UniProt ID: Q9Y4Z0
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: LSM4, YER112W, GRP
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using LSM4 antibody (orb1257266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human mammary cancer using LSM4 antibody (orb1257266) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using LSM4 antibody (orb1257266) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using LSM4 antibody (orb1257266) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of Jurkat cells using 1 ug LSM4 antibody (orb1257266). Western blot was performed from the immunoprecipitate using LSM4 antibody (orb1257266) at a dilution of 1:1000.