LSD1 Antibody (N-term)

SKU: orb30166

Description

Purified rabbit polyclonal antibody.

Research Area

Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100, IF/ICC: 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB7579; RB7580
• Immunogen: Synthetic Peptide
• Target: KDM1A
• Molecular Weight: 92903
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Lysine-specific histone demethylase 1 antibody, anti-Amine oxidase flavin containing domain protein 2 antibody, anti-BRAF35-HDAC complex protein BHC110 antibody, anti-AOF2 antibody, anti-KIAA0601 antibody, anti-KDM1 antibody

Fluorescent confocal image of Hela cell stained with LSD1 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LSD1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). LSD1 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-LSD1 Pab in mouse brain tissue lysate (35 ug/lane). LSD1 (arrow) was detected using the purified Pab.

Western blot analysis of AOF2 (arrow) using LSD1 Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2).

Quick Database Links

Gene Symbol

KDM1A

UniProt

O60341

RefSeq (Protein):NP_001009999.1, NP_055828.2