LRRC33 Rabbit Polyclonal Antibody

• Catalog Number: orb185024
• Category: Antibodies
• Description: LRRC33 Rabbit Polyclonal Antibody
• Target: NRROS
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human LRRC33 (501-600/692aa)
• UniProt ID: Q86YC3
• MW: 74 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ELLP3030; GARPL1; NRROS_HUMAN; Leucine rich repeat
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (LRRC33) Polyclonal Antibody, Unconjugated (orb185024) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868589) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (LRRC33) Polyclonal Antibody, Unconjugated (orb185024) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868589) for 90 minutes, and DAPI for nuclei staining.

Sample: Bone (Rat) Cell Lysate at 40 ug, Thymus (Rat) Cell Lysate at 40 ug, Primary: Anti-LRRC33 (orb185024) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74 kD, Observed band size: 74 kD.