LRRC33 Rabbit Polyclonal Antibody

SKU: orb185024

Description

LRRC33 Rabbit Polyclonal Antibody

Research Area

Neuroscience; Cell Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LRRC33 (501-600/692aa)
• Target: NRROS
• Molecular Weight: 74 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ELLP3030; GARPL1; LRRC33; SENEBAC; UNQ3030; E430025L02Rik; Lrcc33; RGD1310771; LRC33_HUMAN; NRROS; Leucine-rich repeat-containing protein 33; Negative regulator of reactive oxygen species; LRC33_MOUSE; LRC33_RAT; Leucine-rich repeat-containing protein 33^;

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (LRRC33) Polyclonal Antibody, Unconjugated (orb185024) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868589) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (LRRC33) Polyclonal Antibody, Unconjugated (orb185024) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868589) for 90 minutes, and DAPI for nuclei staining.

Sample: Bone (Rat) Cell Lysate at 40 ug, Thymus (Rat) Cell Lysate at 40 ug, Primary: Anti-LRRC33 (orb185024) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74 kD, Observed band size: 74 kD.

Quick Database Links

Gene Symbol

NRROS

UniProt

Q86YC3