LRP/MVP Rabbit Polyclonal Antibody

• Catalog Number: orb11002
• Category: Antibodies
• Description: LRP/MVP Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LRP (88-180/893aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=2μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 99 kDa
• Target: MVP
• UniProt ID: Q14764
• RRID: AB_10752472
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 2310009M24Rik; LRP; Lung Resistance Related Protei
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (LRP/MVP) polyclonal Antibody, Unconjugated (orb11002) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (LRP/MVP) Polyclonal Antibody, Unconjugated (orb11002) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

The figure annotation: The blue histogram is unstained cells. The Orange histogram is cells stained with Rabbit IgG/FITC (orb525556), The green histogram is cells stained with Rabbit Anti-LRP/MVP/FITC Conjugated antibody. Controls, Positive control: HepG 2 cells, Isotype control: Cell lines treated with Rabbit IgG/FITC (orb525556) instead of the primary antibody to confirm that primary antibody binding is specific. 2 µg in 1 00 µl 1 X PBS containing 0.5% BSA.