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Catalog Number | orb1246445 |
---|---|
Category | Antibodies |
Description | LRP4 Antibody |
Target | LRP4 |
Clonality | Polyclonal |
Conjugation | Unconjugated |
Reactivity | Human |
Predicted Reactivity | Bovine, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | 500 ug/mL |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Immunogen | The immunogen for this antibody is: C-SNPSYRTSTQEVK |
UniProt ID | O75096 |
Tested applications | ELISA, IHC, WB |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 200kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 212kDa according to NP_002325.2). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 3.75ug/ml. |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | LRP4, low density lipoprotein receptor-related pro Read more... |
Note | For research use only |
NCBI | NP_002325.2 |
orb1246445 (0.1 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1246445 (3.75 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC, WB | |
Bovine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ICC, IF, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ICC, IF, IHC | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
APC |