LPA2 Rabbit Polyclonal Antibody

SKU: orb184833

Description

LPA2 Rabbit Polyclonal Antibody

Research Area

G Protein-Coupled Receptors, GPCR Signaling Pathway, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LPA2 (1-100/351aa)
• Target: LPAR2
• Molecular Weight: 39 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Endothelial Cell Differentiation Gene 4; Endothelial differentiation lysophosphatidic acid G protein coupled receptor 4; IPA2; LPA receptor 2; LPA receptor EDG4; LPA2; LPAR2; Lysophosphatidic acid receptor Edg4; Edg4; LPAR2_HUMAN.

Blank control: mouse thymouses (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody, dilution: 1 µl in 100 µl 1X PBS containing 0.5% BSA (green).

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-LPA2 antibody (orb184833), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (LPA2) Polyclonal Antibody, Unconjugated (orb184833) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Positive control: (mo) Splenocytes (2% Paraformaldehyde-fixed), Isotype Control Antibody: Rabbit IgG dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Secondary Antibody: Goat anti-rabbit IgG-FITC dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody: rabbit Anti-LPA2 (orb184833) dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA.

Tissue/Cell: rat spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-LPA2 Polyclonal Antibody, Unconjugated (orb184833) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

LPAR2

UniProt

Q9HBW0