LNX2 Rabbit Polyclonal Antibody

SKU: orb184982

Description

LNX2 Rabbit Polyclonal Antibody

Research Area

Basal Lamina, Cell Biology, Lamins

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Canine, Equine, Gallus, Mouse, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LNX2 (401-500/690aa)
• Target: LNX2
• Molecular Weight: 76 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

FLJ12933; FLJ23932; FLJ38000; Ligand of Numb protein X 2; LNX 2; LNX2; LNX2_HUMAN; MGC46315; Numb binding protein 2; Numb-binding protein 2; PDZ domain containing ring finger 1; PDZ domain containing RING finger protein 1; PDZ domain-containing RING finger protein 1; PDZRN 1; PDZRN1.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (LNX2) Polyclonal Antibody, Unconjugated (orb184982) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-LNX2 (orb184982) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 76 kD, Observed band size: 76 kD.

Quick Database Links

Gene Symbol

LNX2

UniProt

Q8N448