Llama IgG1 antibody

• Catalog Number: orb420155
• Category: Antibodies
• Description: Llama IgG1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IP, WB
• Reactivity: Camelus
• Isotype: IgG1
• Immunogen: Llama IgG1
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1 ug/ml, IP: User Optimized, WB: 1:20,000 or 1:40,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Llama IgG1 antibody is directed against Llama IgG1 isotype with minimal cross-reactivity to Llama IgG2 and Llama IgG3. Anti-Llama IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Llama IgG1 and Llama Serum. No reaction was observed against Llama IgG2 and Llama IgG3. Specificity was confirmed by ELISA. For IgG2 and IgG3 reactivity was less than 5% against target (IgG3). This antibody does not recognize recombinant VHH with IgG3 background.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: IgG1 antibody, Llama IgG1, anti-subclass secondary
• Note: For research use only
• Application notes: Antibody Anti-Llama IgG1 has been tested by ELISA, dot blot, and western blot and is suitable for IEP, immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Rabbit anti-Llama IgG1 antibody. Antigen: 1) Llama IgG1 (p/n orb343832), 2) Llama IgG2 (p/n orb343833), 3) Llama IgG3 (p/n orb343834), 4) VHH protein. Load: 10 ng, 3.3 ng, 1.1 ng, and 0.12 ng as indicated. Primary antibody: Llama IgG1 antibody at 1 ug/ml for one hour at RT. Secondary antibody: Gt-a-Rb HRP (p/n orb347654) secondary antibody at 1:40000 for 30 min at RT. Block: (p/n orb348637) overnight at 4°C.

Western Blot of Rabbit Anti-Llama IgG1 antibody. Lane 1: Opal Pre-stained Ladder. Lane 2: Llama IgG1. Lane 3: Llama IgG2 (p/n orb343833). Load: 50 ng per lane. Product: Rabbit Anti-Llama IgG1 at 1:20000. Secondary antibody: Gt-a-Rb HRP (p/n orb347654) at 1:40000 for 30 min at RT. Block: (p/n orb348637) overnight at 4°C. Predicted/Observed size: 50 kDa, 30 kDa for Llama IgG1. Other band(s): none.