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    Llama IgG1 antibody

    Catalog Number: orb420155

    DispatchUsually dispatched within 5-10 working days
    $ 394.00
    Catalog Numberorb420155
    CategoryAntibodies
    DescriptionLlama IgG1 antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsDOT, ELISA, IP, WB
    ReactivityCamelus
    IsotypeIgG1
    ImmunogenLlama IgG1
    Concentration1.0 mg/ml
    Dilution rangeELISA: 1 ug/ml, IP: User Optimized, WB: 1:20,000 or 1:40,000
    Form/AppearanceLiquid (sterile filtered)
    PurityAnti-Llama IgG1 antibody is directed against Llama IgG1 isotype with minimal cross-reactivity to Llama IgG2 and Llama IgG3. Anti-Llama IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Llama IgG1 and Llama Serum. No reaction was observed against Llama IgG2 and Llama IgG3. Specificity was confirmed by ELISA. For IgG2 and IgG3 reactivity was less than 5% against target (IgG3). This antibody does not recognize recombinant VHH with IgG3 background.
    ConjugationUnconjugated
    StorageStore vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesIgG1 antibody, Llama IgG1, anti-subclass secondary
    Read more...
    NoteFor research use only
    Application notesAntibody Anti-Llama IgG1 has been tested by ELISA, dot blot, and western blot and is suitable for IEP, immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.
    Expiration Date12 months from date of receipt.
    Llama IgG1 antibody

    Dot Blot of Rabbit anti-Llama IgG1 antibody. Antigen: 1) Llama IgG1 (p/n orb343832), 2) Llama IgG2 (p/n orb343833), 3) Llama IgG3 (p/n orb343834), 4) VHH protein. Load: 10 ng, 3.3 ng, 1.1 ng, and 0.12 ng as indicated. Primary antibody: Llama IgG1 antibody at 1 ug/ml for one hour at RT. Secondary antibody: Gt-a-Rb HRP (p/n orb347654) secondary antibody at 1:40000 for 30 min at RT. Block: (p/n orb348637) overnight at 4°C.

    Llama IgG1 antibody

    Western Blot of Rabbit Anti-Llama IgG1 antibody. Lane 1: Opal Pre-stained Ladder. Lane 2: Llama IgG1. Lane 3: Llama IgG2 (p/n orb343833). Load: 50 ng per lane. Product: Rabbit Anti-Llama IgG1 at 1:20000. Secondary antibody: Gt-a-Rb HRP (p/n orb347654) at 1:40000 for 30 min at RT. Block: (p/n orb348637) overnight at 4°C. Predicted/Observed size: 50 kDa, 30 kDa for Llama IgG1. Other band(s): none.

    • Control Normal llama IgG1 Antibody [orb1236999]

      ELISA,  FC,  WB

      Camelus

      Unconjugated

      0.5 mg
    • Llama IgG1 isotype control Antibody [orb343832]

      ELISA,  FC,  IF,  SDS-PAGE,  WB

      Unconjugated

      Llama IgG1 isotype control has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain). No bands corresponding to llama IgG2 or IgG3 are observed.

      Llama

      0.5 mg
    • Camelid conserved sequence Antibody (iFluor647) [orb1736345]

      FC,  IF

      Unconjugated

      100 μg
    • Camelid conserved sequence Antibody (iFluor 555) [orb1736346]

      FC,  IF

      Unconjugated

      100 μg
    • Camelid conserved sequence Antibody (HRP) [orb1736347]

      FC,  IF

      Unconjugated

      100 μg
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