Lipoprotein lipase Rabbit Polyclonal Antibody

• Catalog Number: orb13546
• Category: Antibodies
• Description: Lipoprotein lipase Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Guinea pig, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LPL (401-475/475aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg /test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52 kDa
• Target: LPL
• UniProt ID: P06858
• RRID: AB_10749346
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Lipoprotein lipase; LIPD; LIPL_HUMAN; LPL; LPL pro
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Barrett, Helen L. et al. Placental lipase expression in pregnancies complicated by preeclampsia: a case-control study Reprod Biol Endocrinol, 13, 100 (2015)

PubMed: 26336959

Blank control (blue line): raji (fixed with pre-warmed 4% paraformaldehyde for 30 min at 37°C and then permeabilized with 90% ice-cold methanol for 30 min on ice), Primary Antibody (green line): Rabbit Anti-Lipoprotein lipase antibody (orb13546), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Lipoprotein lipase) Polyclonal Antibody, Unconjugated (orb13546) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Lipoprotein lipase (orb13546) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52kD, Observed band size: 55kD.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human HL-60 cell lysates, Primary: Anti-Lipoprotein lipase (orb13546) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kDa, Observed band size: 60 kDa.