Lipocalin 2/NGAL Rabbit Polyclonal Antibody

• Catalog Number: orb11123
• Category: Antibodies
• Description: Lipocalin 2/NGAL Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rabbit, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Lipocalin 2 (101-198/198aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 20 kDa
• Target: LCN2
• UniProt ID: P80188
• RRID: AB_10746402
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Lipocalin 2; Neutrophil Gelatinase associated Lipo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Standage, Stephen Wade et al. NMR-Based Serum and Urine Metabolomic Profile Reveals Suppression of Mitochondrial Pathways in Experimental Sepsis-Associated Acute Kidney Injury Am J Physiol Renal Physiol, (2021)

PubMed: 33843271

Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NGAL Polyclonal Antibody, Unconjugated (orb11123) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.