Lipoamide Dehydrogenase Antibody

SKU: orb344815

Description

Lipoamide Dehydrogenase antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Porcine
• Application Notes:
  Anti-Lipoamide Dehydrogenase has been tested by ELISA and western blot and is assayed against 1.0 ug of Lipoamide Dehydrogenase [Porcine Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:30,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Lipoamide Dehydrogenase [Porcine Heart]
• Purity: Anti-Lipoamide Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart]. Cross reactivity against Lipoamide Dehydrogenase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Lipoamide Dehydrogenase Antibody, Dihydrolipoyl dehydrogenase mitochondrial, Dihydrolipoamide dehydrogenase, Lipoamide Dehydrogenase

Western Blot of Rabbit Anti-Lipoamide Dehydrogenase Antibody. Lane 1: Lipoamide Dehydrogenase Reduced [0.05 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Lipoamide Dehydrogenase Non-Reduced [0.05 µg]. Primary Antibody: Anti-Lipoamide Dehydrogenase at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit IgG [H&L] HRP (p/n orb347673) at 1:40000 for 30 mins at RT. Blocking: BlockOut buffer (p/n orb348644) for 1hr at RT. Expected MW: 55kDa. Exp: 10 secs.

Quick Database Links

UniProt

P09623

RefSeq:118675