LIME antibody

• Catalog Number: orb43903
• Category: Antibodies
• Description: Mouse Monoclonal to LIME.
• Clonality: Monoclonal
• Clone Number: LIME-10
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: LIME1
• Entrez: 54923
• UniProt ID: Q9H400
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-LIME antibody
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml.Western blotting: Reducing conditions; recommended dilution: 1 μg/ml.Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Anti-Hu LIME1 purified antibody (clone LIME-10) specificity verification by flow cytometry. Analysis of the antibody staining was performed on fixed and permeabilized cell suspension. Anti-Hu LIME1 purified antibody (clone LIME-10) (concentration in sample 1 µg/ml, red-filled histogram) binds specifically to intacellular LIME1 in transfected cells (upper panel), but not to Jurkat cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control purified antibody (MOPC-21) under same conditions (concentration in sample 1 µg/ml, black-dashed histogram).

Western blotting analysis of human LIME using mouse monoclonal antibody LIME-10 on lysates of J77-LIME transfectants and Jurkat cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 1 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. LIME was detected around 30 kDa.