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Catalog Number | orb43901 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to LIME. |
Clonality | Monoclonal |
Clone Number | LIME-06 |
Tested applications | FC, IP |
Reactivity | Human |
Isotype | Mouse IgG1 kappa |
Immunogen | Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME. |
Concentration | 1 mg/ml |
Dilution range | Flow cytometry: Recommended dilution: 8-12 μg/ml. Intracellular staining. |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | LIME1 |
Entrez | 54923 |
UniProt ID | Q9H400 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-LIME antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 8-12 μg/ml. Intracellular staining. |
Expiration Date | 12 months from date of receipt. |
Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92; cat. no. orb44416) or anti-human CD4 (MEM-16; cat. no. orb44114), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).
Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-LIME1 (LIME-06) purified antibody (concentration in sample 9 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).
Separation of human CD3 positive LIME1 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-LIME1 (LIME-06) purified antibody (concentration in sample 9 µg/ml, GAM APC).
Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-LIME1 (LIME-06) purified antibody (concentration in sample 9 µg/ml, GAM APC).
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