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Catalog Number | orb1955622 |
---|---|
Category | Proteins |
Description | LIF Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 19.7 kDa and the accession number is P15018-1. |
Tag | Tag Free |
Purity | SDS-PAGE: 99.5%; SEC-HPLC: 98.8% |
UniProt ID | P15018 |
MW | 19.7 kDa (predicted); 23-43 kDa (reducing condition, due to glycosylation) |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | LIF Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 19.7 kDa and the accession number is P15018-1. |
Expression Region | A DNA sequence encoding the human LIF (P15018-1) (Met1-Phe202) was expressed. Predicted N terminal: Ser 23 |
Storage | -20°C |
Note | For research use only |
> 98% as determined by SDS-PAGE and HPLC. | |
19.7 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
19.7 kDa | |
E.coli |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 46 kDa after removal of the signal peptide. The apparent molecular mass of LIF-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |