LIF antibody

• Catalog Number: orb1429401
• Category: Antibodies
• Description: Rabbit polyclonal antibody to LIF.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21725
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P-Leica: 1:500, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 22008
• Target: LIF
• UniProt ID: P15018
• NCBI: NP_001244064.1, NP_002300.1
• Alternative names: Leukemia inhibitory factor, LIF, Differentiation-s
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot:Anti-LIF Antibody (Center) at 1:1000 dilution + Rat spleen lysate.

Western blot:All lanes:Anti-LIF Antibody (Center) at 1:1000 dilution.Lane 1:KG-1;Lane 2:Human spleen.

Immunohistochemical analysis of human kidney tissue using orb1429401 performed on the Leica® BOND RXm at 1:500 dilution.A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Flow cytometric analysis of U-2 OS cells stained with orb1429401(green line) at 1:25 dilution.Secondary antibody was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed.Isotype control antibody (blue line) was rabbit IgG1.