Lgals1 Antibody

• Catalog Number: orb1252358
• Category: Antibodies
• Description: Lgals1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GAL1/1831
• Tested applications: ELISA, FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, lambda
• Immunogen: A human partial recombinant protein corresponding to amino acids 12-108 was used as the immunogen for the Galectin 1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: ELISA: 2-4 ug/ml; order the BSA-free formulation for coatingIF: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTTitering of the Galectin 1 antibody may be required for optimal performance.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Lgals1
• UniProt ID: P09382
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Galectin-1, Gal-1, 14 kDa laminin-binding protein,
• Note: For research use only
• Application notes: ELISA: 2-4 ug/ml; order the BSA-free formulation for coatingIF: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTTitering of the Galectin 1 antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human prostate carcinoma with Galectin 1 antibody (clone GAL1/1831). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Western blot testing of human 1) HeLa, 2) K562 and 3) HEK293 cell lysate with Galectin 1 antibody (clone GAL1/1831). Expected molecular weight ~14 kDa.