Galectin 1/LGALS1 Antibody

• Catalog Number: orb334576
• Category: Antibodies
• Description: Galectin 1/LGALS1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Galectin 1 recombinant protein (Position: A2-D135). Human Galectin 1 shares 88% and 90% amino acid (aa) sequence identity with mouse and rat Galectin 1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human ELISA , 0.1-0.5μg/ml, Human, -
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 14716 MW
• UniProt ID: P09382
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Galectin-1;Gal-1;14 kDa laminin-binding protein;HL
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of PC-3 cells using anti-Galectin 1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Galectin 1 using anti-Galectin 1 antibody.Lane 1:rat testis tissue;2:mouse spleen tissue;3:HELA cell;4:SMMC cell.

IF analysis of Galectin 1 using anti-Galectin 1 antibody. Galectin 1 was detected in immunocytochemical section of U20S cells.

IHC analysis of Galectin 1 using anti-Galectin 1 antibody. Galectin 1 was detected in a paraffin-embedded section of mouse intestine cancer tissue.

IHC analysis of Galectin 1 using anti-Galectin 1 antibody. Galectin 1 was detected in a paraffin-embedded section of rat kidney tissue.

IHC analysis of Galectin 1 using anti-Galectin 1 antibody. Galectin 1 was detected in a paraffin-embedded section of human placenta tissue.