Leptin tA (PEG) Protein

SKU: orb426850

Description

Recombinant of Leptin tA (PEG) protein

Research Area

Metabolism Research

Images & Validation

• Application Notes:
  Protein content: Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics)

Key Properties

• Source: Escherichia coli
• Biological Activity: Leptin Antagonist Triple Mutant Rat Recombinant half-life in circulation after SC injection was over 20 hours.Leptin Antagonist Triple Mutant Rat Recombinant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Leptin Antagonist Triple Mutant Rat Recombinant in vitro activity is 5-6 fold lower than the non-pegylated antagonist, though in vivo it has profound weight gain effect (as compared to the non-pegylated antagonist), resulting mainly from increased food intake.
• Purity: Greater than 99.0% as determined by:(a) Gel filtration analysis.(b) Analysis by SDS-PAGE.

Storage & Handling

• Storage: Stability: Lyophilized Leptin Antagonist Triple Mutant Rat Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested.Please prevent freeze-thaw cycles
• Form/Appearance: White lyophilized (freeze-dried) powder.
• Buffer/Preservatives: The Rat Leptin triple anatagonist was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only