Leptin Salamander Protein

SKU: orb426843

Description

Recombinant of Leptin Salamander protein

Images & Validation

• Application Notes:
  Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.104 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics)

Key Properties

• Source: Escherichia Coli
• Biological Activity: By inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its activity in that test is similar to that of mouse leptin (see Salomon et al (2006) Protein Expression and Purifcation 47, 128–136).
• Protein Sequence: The sequence of the ten N-terminal amino acids was determined and was found to be Ala-Ile-Met-Val-Asp-Gln-Leu-Arg-Met-Asp
• Purity: Greater than 99.0% as determined by: (a) Analysis by Gel-Filtration. (b) Analysis by SDS-PAGE.

Storage & Handling

• Storage: Stability: Lyophilized Salamander Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Buffer/Preservatives: The Salamander Leptin was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
• Disclaimer: For research use only

Alternative Names

OB Protein, Obesity Protein, OBS, Obesity factor, Leptin.