Leptin receptor Rabbit Polyclonal Antibody

• Catalog Number: orb6313
• Category: Antibodies
• Description: Leptin receptor Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Bovine, Canine, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Leptin receptor (901-1000/1165aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 126 kDa
• Target: LEPR
• UniProt ID: P48357
• RRID: AB_10930656
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: obl; CD 295; CD295; CD295 antigen; Db; Fa; HuB219;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (Black line): Raji (Black). Primary Antibody (green line): Rabbit Anti-Leptinreceptor antibody (orb6313), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ice-cold methanol overnight at 4°C and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-Leptin receptor antibody (orb6313), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Cell: (mo) Hepatocyte (2% BSA at 4°blocked for 30 minutes. 1% Paraformaldehyde fixed for 30 minutes, 0.1% TritonX-100 permeablized for 2 minutes). Concentration: 1:100. Incubation: 40 minutes. Host/Blank: Hepatocytes. Flow cytometric analysis of Rabbit Anti-Leptin receptor (long)/AF488 Conjugated antibody (green) compared with control in the absence of primary antibody (blue) followed by (mo)hepatocytes.