CD47 Rabbit Polyclonal Antibody (Carrier-free)

SKU: orb3118803

Description

Anti-CD47 Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Research Area

Cancer Biology, Disease Biomarkers, Immunology & Inflammation, Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Mouse Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
• Reactivity: Human, Mouse
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CD47, which shares 61.8% and 66.7% amino acid (aa) sequence identity with mouse and rat CD47, respectively.
• Target: Leukocyte surface antigen CD47
• Molecular Weight: 50 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD47; CD47 molecule; IAP; Leukocyte surface antigen CD47; MER6; OA3; Protein MER6

Quick Database Links

Gene Symbol

Leukocyte surface antigen CD47

UniProt

Q08722