Leptin Antibody

• Catalog Number: orb1539107
• Category: Antibodies
• Description: Leptin Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1,k
• Immunogen: E.coli derived recombinant Human Leptin.
• Concentration: 1 mg/ml
• Dilution range: ELISA (12 - 16 µg/ml), IHC, IHC-P (10 µg/ml), WB (0.2 - 0.4 µg/ml)
• Conjugation: Unconjugated
• Target: Leptin
• Storage: Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from PBS. Lyophilized from PBS
• Alternative names: LEP, Leptin, LEPD, Obese protein, Obese, mouse, ho
• Note: For research use only
• Application notes: Resuspension: Reconstitute with sterile water. Further information: Immunohistochemistry: LS-B8192 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect recombinant human Leptin when used with biotinylated antigen affinity purified anti-human Leptin as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 2-4 ng/lane, under non-reducing conditions.
• Expiration Date: 12 months from date of receipt.