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Catalog Number | orb1524745 |
---|---|
Category | Antibodies |
Description | LEF1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | between 349 and 399 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | LEF1 |
UniProt ID | Q9UJU2 |
NCBI | NP_057353.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | T cell-specific transcription factor 1-alpha; TCF7 Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human LEF1 by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-LEF1 antibody orb1524745 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. LEF1 was also immunoprecipitated by rabbit anti-LEF1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Detection of human LEF1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-LEF1 (Cat. No. orb1524745) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
FC, WB | |
Bovine, Canine, Gallus, Porcine, Rabbit, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IHC-Fr, IHC-P, IP, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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