LEF1 antibody

• Catalog Number: orb13540
• Category: Antibodies
• Description: LEF1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LEF-1 (331-399/399 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 44 kDa
• Target: LEF-1
• UniProt ID: Q9UJU2
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: lymphoid enhancing factor-1; Transcription factor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin embedded mouse skin tissue using LEF1antibody

Paraffin-embedded rat brain tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.).LEF1 antibody at 1:200 dilution with a overnight incubation period

IHC-P of mouse tissue (LEF1 antibody at 1:200)

IHC-P of rat liver tissue (LEF1 antibody at 1:300)

Immunohistochemical analysis of formalin fixed and paraffin embedded human colon carcinoma using LEF1 antibody