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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | (1) Measured by its binding ability in a functional ELISA. Immobilized Human LDL R, His Tag at 10 ug/mL (100 ul/well) can bind Biotinylated Human PCSK9 with a linear range of 0.01-0.1 ug/mL. (2) Measured by its binding ability in a functional ELISA. Immobilized Human LDL R, His Tag at 10 ug/mL (100 ul/well) can bind Biotinylated Human (D374Y) PCSK9 with a linear range of 0.02-0.5 ug/mL. |
| Target | LDLR |
| Tag | His Tag |
| MW | 86 kDa |
| Purity | >90% as determined by SDS-PAGE. |
| Protein Sequence | Ala 22 - Arg 788 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human LDL R Protein (Biotin) [orb2994702]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 88.4 KDa. Observed: 95-140 KDa, reducing conditions
20 μg, 100 μgRecombinant Human LDL R Protein [orb2994788]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 86.56 KDa. Observed: 99-135 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human LDLRAD3 Protein, N-Fc [orb2963420]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
44.61 kDa
1 mg, 50 μg, 100 μg

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The purity of rh LDLR was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_000518.1 |
|---|
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Protocol Information
LDLR Recombinant Protein (orb1227968)
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