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Catalog Number | orb1248197 |
---|---|
Category | Antibodies |
Description | LDHB Antibody |
Target | LDHB |
Clonality | Polyclonal |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Porcine, Rat |
Predicted Reactivity | Bovine, Canine |
Form/Appearance | Liquid |
Concentration | 500 ug/mL |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Immunogen | The immunogen for this antibody is: C-NARGLTSVINQKLK |
UniProt ID | P07195 |
MW | Approx 35kDa |
Tested applications | ELISA, WB |
Application notes | Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 35kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 36.6kDa according to NP_002291.1). Recommended concentration: 0.03-0.1ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | LDHB, lactate dehydrogenase B, LDH-B, LDH-H, LDHBD Read more... |
Note | For research use only |
NCBI | NP_002291.1 |
orb1248197 (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1248197 (0.1 ug/ml) staining of Pig Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
IHC-P, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |