You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1246770 |
---|---|
Category | Antibodies |
Description | LDB3 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Canine, Mouse, Porcine, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-KSQNKPEDEADE |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Western Blot experiments gave bands at approx 90kDa and 35kDa in Human Skeletal Muscle lysate after 1ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Huang et al J Biol Chem. 2003 Feb 28;278(9):7360-5; PMID: 12499364). This protein has a calculated MW of 77.1kDa according to NP_009009.1. Recommended concentration: 0.1-1ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | LDB3 |
UniProt ID | O75112 |
NCBI | NP_001073584.1, NP_001073585.1, NP_009009.1, NP_001073583.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | LDB3, ZASP, LIM domain binding 3 , HGNC:15710, KIA Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Western Blot experiments gave bands at approx 90kDa and 35kDa in Human Skeletal Muscle lysate after 1ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Huang et al J Biol Chem. 2003 Feb 28;278(9):7360-5; PMID: 12499364). This protein has a calculated MW of 77.1kDa according to NP_009009.1. Recommended concentration: 0.1-1ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1246770 (0.1 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1246770 (3.8 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
Filter by Rating