LC3A/B Rabbit Polyclonal Antibody

• Catalog Number: orb499603
• Category: Antibodies
• Description: LC3A/B Rabbit Polyclonal Antibody
• Target: MAP1LC3B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Goat, Guinea pig, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human LC3A/B (1-50/121aa)
• UniProt ID: Q9GZQ8
• MW: 14 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MLP3A_HUMAN; Microtubule-associated proteins 1A/1B
• Research Area: Active Protein-Gene, Autophagy, Cell Cycle, Metabo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Hela (fixed with 70% ethanol (Overmight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min at -20°C). Primary Antibody (green line): Rabbit Anti-LC3A/B antibody (orb499603), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (LC3A/B) Polyclonal Antibody, Unconjugated (orb499603) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.