LC3A/B Rabbit Polyclonal Antibody

SKU: orb157781

Description

LC3A/B Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LC3A/B (31-121/121aa)
• Target: Map1lc3b
• Molecular Weight: 14 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; 1010001C15Rik; Atg8; MAP1A/MAP1B; Map1lc3; Mpl3; zbs559; MLP3B_HUMAN; MAP1LC3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; Microtubule-associated proteins 1A/1B light chain 3B (MAP1A/MAP1B LC3 B | MAP1A/MAP1B light chain 3 B); MAP1ALC3; MLP3B_MOUSE; MLP3B_RAT;

Tissue/Cell: mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-LC3A/B Polyclonal Antibody, Unconjugated (orb157781) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

Map1lc3b

UniProt

Q9GZQ8