LC3 Rabbit Polyclonal Antibody

• Catalog Number: orb500748
• Category: Antibodies
• Description: LC3 Rabbit Polyclonal Antibody
• Target: MAP1LC3A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Equine, Human, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human LC3 (31-121/121aa)
• UniProt ID: Q9H492
• MW: 14/16 kDa
• Tested applications: WB
• Dilution range: WB=1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Autophagy related protein LC3 A; Autophagy related
• Note: For research use only

References

Sharmistha Samanta , Gopinath Mukherjee , Sreya Chattopadhyay , Biswadev Bishay Concomitant inhibition of TLR4 and NLRP3 inflammasome in macrophages regulates lipopolysaccharide induced inflammation The Microbe, (2025)

PubMed:

Applications

WB

Reactivity

Mouse

Protein: mouse brain lysates at 40 ug, Primary: rabbit Anti-LC3 (orb500748) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 14 kD Observed band size: 14, 16 kD.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebellum (Mouse) Lysate at 40 ug, Lane 3: Testis (Mouse) Lysate at 40 ug, Primary: Anti-LC3 (orb500748) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17/14 kD, Observed band size: 17 kD.