LC3 Rabbit Polyclonal Antibody

SKU: orb500748

Description

LC3 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Equine, Human, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LC3 (31-121/121aa)
• Target: MAP1LC3A
• Molecular Weight: 16 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3; 1010001H21Rik; 4922501H04Rik; LC3-I; LC3-II; MLP3A_HUMAN; MAP1LC3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; Microtubule-associated proteins 1A/1B light chain 3A (MAP1A/MAP1B LC3 A | MAP1A/MAP1B light chain 3 A); MLP3A_MOUSE; MLP3A_RAT;

Protein: mouse brain lysates at 40 ug, Primary: rabbit Anti-LC3 (orb500748) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 14 kD Observed band size: 14, 16 kD.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebellum (Mouse) Lysate at 40 ug, Lane 3: Testis (Mouse) Lysate at 40 ug, Primary: Anti-LC3 (orb500748) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17/14 kD, Observed band size: 17 kD.

Quick Database Links

Gene Symbol

MAP1LC3A

UniProt

Q9H492

Sharmistha Samanta , Gopinath Mukherjee , Sreya Chattopadhyay , Biswadev Bishay Concomitant inhibition of TLR4 and NLRP3 inflammasome in macrophages regulates lipopolysaccharide induced inflammation The Microbe, (2025)

PubMed:

Applications

WB

Reactivity

Mouse