LC3 Mouse Monoclonal Antibody

• Catalog Number: orb783388
• Category: Antibodies
• Description: LC3 Mouse Monoclonal Antibody
• Target: MAP1LC3B
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,k
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Recombinant protein of human MAP1LC3A/B
• UniProt ID: Q9GZQ8
• MW: 13 kDa
• Tested applications: WB
• Dilution range: WB=1:500-1000
• Antibody Type: Primary Antibody
• Clone Number: L3G1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MLP3A_HUMAN; Microtubule-associated proteins 1A/1B
• Note: For research use only

Sample: Human Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Primary: Anti-LC3 (orb783388) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 13 kD, Observed band size: 13 kD.

Sample: Lane 1: Brain (Mouse) Tissue Lysate, Lane 2: Brain (Rat) Tissue Lysate, Lane 3: Brain (Human) Tissue Lysate, Primary: Anti-LC3 (orb783388) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 13 kD, Observed band size: 17 kD.

Sample: Lane 1: Y79 (soluble fraction of cell extract) cell lysate, Lane 2: 293 transfected with human LC3 (whole cell extract) cell lysate, Primary: Anti-LC3 (orb783388) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 13 kD, Observed band size: 18/16 kD.