LASS1 Antibody

• Catalog Number: orb1246449
• Category: Antibodies
• Description: LASS1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Mouse, Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KDLREYDTAEAQ
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: LASS1
• UniProt ID: P27544
• NCBI: NP_937850.1, NP_067090.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: LAG1, CerS1, LASS1, LAG1 homolog, ceramide synthas
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 37 kDa band observed in Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates (calculated MW of 38.0 kDa according to NP_937850.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 85 kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Expiration Date: 12 months from date of receipt.

orb1246449 (1 ug/ml) staining of Frontal Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.