LAR Rabbit Polyclonal Antibody

SKU: orb157775

Description

LAR Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=1μg/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PTPRF (30-100/1907aa)
• Target: PTPRF
• Molecular Weight: 210 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LAR; LAR protein; LARFN5C; PTPRF; LARS; LCA homolog; Leukocyte antigen related(LAR) PTP receptor; Leukocyte antigen related; Leukocyte antigen related PTP receptor; Leukocyte antigen related tyrosine phosphatase; Leukocyte common antigen related; Protein Tyrosine Phosphatase Receptor Type F; Protein tyrosine phosphatase receptor type F polypeptide; Ptprf; PTPRF protein; PTPRF_HUMAN; Receptor linked protein tyrosine phosphatase LAR; Receptor type tyrosine protein phosphatase F; Receptor type tyrosine protein phosphatase F precursor; Receptor-type tyrosine-protein phosphatase F.

Blank control (blue): Hela Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-LAR/AF488 Conjugated antibody, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions.

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-LAR antibody (orb157775), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

PTPRF

UniProt

P10586