LAPTM5 Rabbit Polyclonal Antibody

SKU: orb184851

Description

LAPTM5 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=2ug/Test
• Reactivity: Human
• Predicted Reactivity: Equine, Gallus, Mouse, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LAPTM5 (101-200/262aa)
• Target: LAPTM5
• Molecular Weight: 30 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

LAPM5_HUMAN; LAPTM5; Lysosomal-associated multitransmembrane protein 5; Lysosomal-associated transmembrane protein 5; Retinoic acid-inducible E3 protein.

Blank control (black line): U937. Primary Antibody (green line): Rabbit Anti-LAPTM5 antibody (orb184851), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

LAPTM5

UniProt

Q13571

References

Yu Chen 1 2 3, Shanshan Zhu 1, Tianxing Liao 1, Chunxuan Wang 1, Jiajun Han 1, Zhenyu Yang 1, Xiaolong Lu 1 2 3, Zenglei Hu 2 4, Jiao Hu 1 2 3, Xiaoquan Wang 1 2 3, Min Gu 1 2 3, Ruyi Gao 1 2 3, Kaituo Liu 2 4, Xiaowen Liu 1 2 3, Chan Ding 2 5, Shunlin Hu 1 2 3, Xiufan Liu The HN protein of Newcastle disease virus induces cell apoptosis through the induction of lysosomal membrane permeabilization PLoS Pathog, 20(2), e1011981 (2024)

PubMed: 38354122

Applications

WB

Reactivity

Human

Chenghao Fan 1, Shen Song 2, Yu Han 2, Dongcheng Cai 2, Anteng Shi 2, Fangfang Wan 2, Jie Feng 2, Jiajun Zhong 2, Yifan Xie 2, Fei Xu 2, Jiangping Song 3, Shengshou Hu 4, Yu Nie 5, Hui Zhang 6 Targeting lysozyme 2 in endocardium promotes rapid recovery by modulating remote injury signals Cell Stem Cell ., (2025)

PubMed: 40967223

Applications

IHC

Reactivity

Mouse

Zhao, Li et al. Vpr counteracts the restriction of LAPTM5 to promote HIV-1 infection in macrophages Nat Commun, 12, 3691 (2021)

PubMed: 34140527

Applications

WB

Reactivity

Human