LAPTM5 Rabbit Polyclonal Antibody

• Catalog Number: orb184851
• Category: Antibodies
• Description: LAPTM5 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Equine, Gallus, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LAPTM5 (101-200/262aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=2ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 30 kDa
• Target: LAPTM5
• UniProt ID: Q13571
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: LAPM5_HUMAN; LAPTM5; Lysosomal-associated multitra
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Yu Chen 1 2 3, Shanshan Zhu 1, Tianxing Liao 1, Chunxuan Wang 1, Jiajun Han 1, Zhenyu Yang 1, Xiaolong Lu 1 2 3, Zenglei Hu 2 4, Jiao Hu 1 2 3, Xiaoquan Wang 1 2 3, Min Gu 1 2 3, Ruyi Gao 1 2 3, Kaituo Liu 2 4, Xiaowen Liu 1 2 3, Chan Ding 2 5, Shunlin Hu 1 2 3, Xiufan Liu The HN protein of Newcastle disease virus induces cell apoptosis through the induction of lysosomal membrane permeabilization PLoS Pathog, 20(2), e1011981 (2024)

PubMed: 38354122

Applications

WB

Reactivity

Human

Zhao, Li et al. Vpr counteracts the restriction of LAPTM5 to promote HIV-1 infection in macrophages Nat Commun, 12, 3691 (2021)

PubMed: 34140527

Applications

WB

Reactivity

Human

Blank control (black line): U937. Primary Antibody (green line): Rabbit Anti-LAPTM5 antibody (orb184851), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.