Langerin Antibody / CD207

• Catalog Number: orb1824042
• Category: Antibodies
• Description: Langerhans cells (LCs) are a subset of immature dendritic cells (DCs) that specifically localize in the epidermis and other mucosal epithelia. Epidermal LCs possess strong immuno-stimulatory capacity and play a central role in the initiation and regulation of immune responses. Langerin (CD207) is a Ca2+-dependent, C-type lectin domain containing, type II transmembrane protein that induces epidermal LCs to differentiate into Birbeck granules (BG). BGs are organelles with superimposing and zippering membranes that influence proper class I type antigen presentation to the circulating T cells. Human spleen, lymph node, thymus, liver, lung and heart express Langerin protein.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant partial protein sequence (within amino acids 50-250) from the human protein was used as the immunogen for the Langerin antibody.
• UniProt ID: Q9UJ71
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: LGRN/7430
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Langerin antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human skin tissue with Langerin antibody (clone LGRN/7430). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human skin tissue with Langerin antibody (clone LGRN/7430). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human skin tissue with Langerin antibody (clone LGRN/7430). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Langerin antibody (clone LGRN/7430) as confirmation of integrity and purity.