LAMP5 Antibody / Lysosome-Associated Membrane Glycoprotein 5

• Catalog Number: orb1824139
• Category: Antibodies
• Description: Lysosome-associated membrane proteins (LAMP) are glycosylated type I membrane proteins that play a role in the biogenesis of the pigment melanin. LAMP-5, also known as C20orf103, is a 280 amino acid protein that belongs to the LAMP family and is mainly found in cytoplasmic vesicles of the neuronal cell body. LAMP-5 is up-regulated upon CpG activation and down-regulated up Toll-like receptor (TLR) ligands.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LAMP5/7642
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2, kappa
• Immunogen: Recombinant full-length human LAMP5 protein was used as the immunogen for the LAMP5 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This LAMP5 antibody is available for research use only.
• UniProt ID: Q9UJQ1
• Storage: Aliquot the LAMP5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot testing of human U937 cell lysate with LAMP5 antibody (clone LAMP5/7642). Predicted molecular weight ~31 kDa, but may be observed at higher molecular weights due to glycosylation.

IHC staining of FFPE human kidney tissue with LAMP5 antibody (clone LAMP5/7642). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free LAMP5 antibody (clone LAMP5/7642) as confirmation of integrity and purity.