Laminin alpha 4 Rabbit Polyclonal Antibody

• Catalog Number: orb2989
• Category: Antibodies
• Description: Laminin alpha 4 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Rabbit, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Laminin alpha 4 (901-1000/1823aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: ICC/IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 200 kDa
• Target: LAMA4
• UniProt ID: Q16363
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: LAMA3; LAMA4 1; LAMA4; LAMA4_HUMAN; Laminin alpha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Tissue/Cell: HeLa cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Laminin alpha 4) Polyclonal Antibody, Unconjugated (orb2989) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.