Lamin B1 Rabbit Polyclonal Antibody

SKU: orb13544

Description

Lamin B1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: WB
• Dilution range: WB=1:500-2000
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Mouse, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Lamin B (361-460/586aa)
• Target: LMNB1
• Molecular Weight: 64 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

LMNB1_HUMAN; Lamin-B1; LMNB1; LMN2; LMNB; LMN; ADLD; MCPH26;

Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-Lamin B antibody (orb13544), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Brain (Rat) lysate at 30 ug, Primary: Anti-Lamin B (orb13544) at 1:200 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 64kD, Observed band size: 64kD.

Quick Database Links

Gene Symbol

LMNB1

UniProt

P20700