Lambda light chain antibody

• Catalog Number: orb1474175
• Category: Antibodies
• Description: Mouse monoclonal antibody to Lambda light chain
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: This antibody is generated from mouse immunized with Lambda light chain recombinant protein.
• Dilution range: WB (1/500 - 1/1000), IH (1/20 - 1/50)
• Form/Appearance: Mouse IgG2a kappa. Liquid in PBS, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: IGLV1-51
• Entrez: 28820
• UniProt ID: P01701
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG2a kappa. Liquid in PBS, and 0.01% sodium azide.
• Alternative names: Immunoglobulin lambda variable 1-51; Ig lambda cha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lambda light chain expression in Ramos (A) whole cell lysates. (Predicted band size: 12 kD; Observed band size: 27 kD)

Immunohistochemical analysis of Lambda light chain staining in human lymph formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.