Lambda (L chain) antibody (FITC)

• Catalog Number: orb43601
• Category: Antibodies
• Description: Mouse Monoclonal to Lambda (L chain).
• Clonality: Monoclonal
• Clone Number: 4C2
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: Ig Lambda Light Chain
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Lambda light chains antibody
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human Ig lambda light chain (4C2) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human Ig lambda light chain positive B-lymphocytes (red-filled) from Ig lambda light chain negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig lambda light chain (4C2) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Ig lambda light chain (4C2) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).