LAG3 Rabbit Polyclonal Antibody

SKU: orb500953

Description

LAG3 Rabbit Polyclonal Antibody

Research Area

CD Markers, Cell Biology, Immunology, Secreted Molecules, Stem Cell Research

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=1μg /test
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Equine, Human, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LAG-3 (201-300/525aa)
• Target: LAG3
• Molecular Weight: 70 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lymphocyte activation gene 3 protein; Lymphocyte activation gene 3; CD223; FDC protein; LAG 3; LAG-3; LAG3_HUMAN.

Blank control: Ctll-2. Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (orb500953), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Ctll-2. Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (orb500953), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: 293T Cell (Human) Lysate at 40 ug, Primary: Anti-LAG-3 (orb500953) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.

Sample: K562 Cell (Human) Lysate at 40 ug, Primary: Anti-LAG-3 (orb500953) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.

Quick Database Links

Gene Symbol

LAG3

UniProt

P18627