Lac I Antibody

SKU: orb345637

Description

Lac I antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:90,000 – 1:100,000, WB: 1:500 - 1:2,000
• Reactivity: Bacteria, E. coli
• Application Notes:
  Rabbit anti-Lac-I antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein. Specific conditions for reactivity and detection of Lac I should be optimized by the end user. Expect a band approximately ~38 kDa in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant Lac I protein.
• Purity: This Anti-Lac I product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence. Cross-reactivity with Lac I from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-LacI antibody, rabbit anti-Lac-I antibody, Rabbit anti-Lac I antibody, Lactose operon repressor, DNA binding transcriptional repressor antibody, Transcriptional repressor of the lac operon antibody

Western Blot of Rabbit Anti-Lac I Antibody. Lane 1: Molecular Weight Marker (3 µl). Lane 2: recombinant LAC-I (0.05 µg). Primary Antibody: Anti-LAC-I at 1:1000 for 1hr at RT. Secondary Antibody: Goat anti-Rabbit IgG HRP (p/n orb347654) at 1:40000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644) for 30 mins at RT. Predicted/Observed MW: ~38kDa.

Western blot using Biorbyt's affinity purified anti-Lac I antibody shows detection of a 38 kDa band corresponding to recombinant Lac I (arrowhead). The blot was blocked with 5% BLOTTO (p/n orb348624) in TTBS overnight at 4°C. Primary antibody was used at a 1:1000 dilution followed by reaction with a 1:10000 dilution of HRP goat anti-rabbit IgG. ECL was used for detection.

Quick Database Links

UniProt

P03023

RefSeq:146576