L-Asparaginase Antibody

SKU: orb344827

Description

L-Asparaginase antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution range: ELISA: 1:5,000 - 1:25,000, IP: 1:100, WB: 1:500 - 1:3,000
• Reactivity: E. coli
• Application Notes:
  Anti-L-Asparaginase antibody has been tested by ELISA and western blot and is assayed in western blot and against 1.0 ug of Asparaginase [E. coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:400,000 is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Asparaginase [E. coli]
• Purity: Anti-L-Asparaginase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Asparaginase [E. coli]. Cross reactivity against Asparaginase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/ml
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-L-Asparaginase Antibody, L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody

Western Blot of Rabbit anti-L-Asparaginase Antibody. Lane 1: L-Asparaginase. Lane 2: none. Load: 100 ng per lane. Primary antibody: L-Asparaginase antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 32 kDa for L-Asparaginase. Other band(s): L-Asparaginase splice variants and isoforms.

Quick Database Links

UniProt

P00805

RefSeq:P00805.2

References

José Gabriel da Silva Gomes 1, Larisse Cadeira Brandão 2, Daniel Pascoalino Pinheiro 3, Larissa Queiroz Pontes 3, Rômulo Farias Carneiro 4, Bárbara Cibelle Soares Farias Quintela 3, Anna Carolina Machado Marinho 3, Gilvan Pessoa Furtado 3, Bruno Anderson Matias Rocha Kinetics characterization of a low immunogenic recombinant l-asparaginase from Phaseolus vulgaris with cytotoxic activity against leukemia cells Int J Biol Macromol, (2024)

PubMed: 38986978

Applications

WB

Reactivity

E. coli