Ku70 + Ku80 Antibody

• Catalog Number: orb385931
• Category: Antibodies
• Description: Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system. In addition to its role in NHEJ, Ku is also required for telomere length maintenance and subtelomeric gene silencing. Ku was originally identified when patients with systemic lupus erythematosus were found to have high levels of autoantibodies to the protein. [Wiki]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LKAP1-1
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A human cell nuclear fraction was used as the immunogen for this Ku70 + Ku80 antibody.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein G purified
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Ku70 + Ku80 antibody is available for research use only.
• Entrez: 2547
• Storage: Store the Ku70 + Ku80 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the Ku70 + Ku80 antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of K562 cells using Ku70+Ku80 antibody