Ku70 + Ku80 Antibody

• Catalog Number: orb248366
• Category: Antibodies
• Description: This antibody recognizes a dimer of two proteins of 70kDa (Ku70) and ~80kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KU729
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence/Immunocytochemistry: 1-2ug/ml for 30 min at RT
• Purity: Protein G purified monoclonal antibody
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Ku70 + Ku80 antibody is available for research use only.
• Entrez: 2547
• Storage: Store the Ku70 + Ku80 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

FACS staining of K562 cells using Ku70+Ku80 antibody