ORF57 Antibody

SKU: orb345631

Description

KSHV ORF57 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:100,000 – 170,000, WB: 1:5,000 - 1:8,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band 50-55 kDa in size corresponding to KSHV ORF57 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human KSHV ORF57 protein.
• Target: ORF57
• Purity: This affinity purified antibody is directed against human herpesvirus 8 (KSHV ORF57) protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with ORF57 protein from human herpesvirus 8 types P and M sources based on 94% homology with the immunizing sequence. Reactivity against homologues from other sources is unknown.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.55 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-KSHV ORF57 antibody, MTA, human herpesvirus 8 protein

Western blot using Biorbyt's affinity purified anti-KSHV ORF57 to detect KSHV ORF57 in HEK293 cells transfected with ORF57 expression vector and ORF57 truncations, or in KSHV infected B-cell line (BCBL-1) treated with or without valproic acid to induce viral replication (arrow). The membrane was probed with the primary antibody diluted 1:7500 (left) and 1:1000 (right).

Quick Database Links

Gene Symbol

ORF57

UniProt

Q2HR75

RefSeq:YP_001129410.1